SERVICES
We can assist you with the creativity and the experience of our skilled chemists to expand your own R&D effort
A continuous range of problem-solving expertise in chemical synthesis makes C4T an ideal partner for both Process Research & Development and Drug Discovery activities. C4T has set long term partnerships with leading Italian Pharmas for Drug Discovery Services and Process Research & Development. Custom synthesis services are provided to big Pharmas as well.
ORGANIC SYNTHESIS
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ROUTE DEVELOPMENT
NMR STUDIES
&
CHEMICAL ANALYSIS
MEDICINAL CHEMISTRY
ORGANIC SYNTHESIS
We provide efficient contract synthesis to produce high-quality, high-purity complex organic molecules in milligram to multi-gram quantities:
- PHARMA & AGRO API IMPURITIES
- Synthesis of qualified API impurities as analytical standards (ansamycines, betalactames, purine nucleosides, ketoprofen, SCMC, Levodropropizine and more)
- Isolation, structure elucidation and route development of unknown impurities
- Single point chemical modification and isotope synthesis for the validation of new structures
- Advanced NMR studies (link to section)
- ORIGINAL SCAFFOLDS/BUILDING BLOCKS
- from heterocycle chemistry to thiopyrophosphate synthesis know-how
- REFERENCE COMPOUNDS
Organic synthesis at C4T accounts for the attitude of problem solving. We long for the most demanding tasks. We explore novel chemistry and synthetic routes by exploiting up-to-date search engines and a distinctive know-how in organic chemistry. We can help you with patents, strategies, evaluation of prior art.
Purification strategies are evaluated on a case-by-case basis including several techniques (e.g., silica/alumina column chromatography, preparative HPLC, ion-exchange, etc.).
Detailed structure elucidation is provided by combining HPLC-MS analysis with complete NMR studies. Most suitable NMR techniques are selected on a case-by-case basis according to complexity of the task. (“NMR Studies & Chemical Analysis” )
Our staff has experience in developing projects up to 100 g scale in our research laboratories, transferring know-how and expertise to our customers and helping them in the first steps of scale-up process.
Documentation
All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.
NMR STUDIES & CHEMICAL ANALYSIS
These services include:
- isolation and STRUCTURE ELUCIDATION OF UNKNOWN API IMPURITIES from process mixtures
- structure elucidation of compounds and mixtures of compounds
- analytical methods development and validation (ICH guidelines)
- qNMR for absolute concentration determination of test items (APIs, API impurities, working standards, etc..)
- analytical characterization of natural and semi-synthetic polysaccharides
High-quality investigations of purity and/or structure of compounds are performed combining several techniques (e.g., NMR, GC-MS, HPLC-MS, HPLC-RID, UV, GPC-RID).
We have developed an Advanced NMR Platform (Bruker 700 MHz) able to address several tasks, taking advantage from the Research Centre of NMR Spectroscopy of “Tor Vegata University”, directed by Prof. D. Cicero, and thanks to a long lasting cooperation with Prof. M. Paci, who devoted a whole career to NMR studies of biological systems.
The platform includes:
- Mono-dimensional NMR: 1H; 13C
- Two-dimensional NMR: TOCSY, NOESY, ROESY, HSQC, HMBC
- qHNMR studies
- Diffusion Ordered Spectroscopy (DOSY) focused on:
- Mixture Analysis
- Characterization of Complexes
- Characterization of polysaccharide derivatives
Documentation
All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.
Analytical Method Validation Protocols, Validation Protocol Reports, Analytical Method Procedures and Analysis Reports are released according to the specific service provided.
MEDICINAL CHEMISTRY
Organic synthesis
- Synthesis of focused libraries solution and solid phase parallel synthesis
- Synthesis of original scaffolds
- Strategy design for hit expansion and optimisation
- Scale up and synthesis of lead compounds
Computational studies
We will help you, hand in hand, in developing your hit identification and hit-to-lead programs in close collaboration with Prof. Orazio Nicolotti’s team (University of Bari – Aldo Moro)
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Documentation
All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.
C4T has matured great experience in specific research areas (i.e., Endocannabinoids, Metalloproteinases, Kinases) driving and developing early stage discovery projects through the closest cooperation with Biology and Medicine Departments of excellence (Academia/Research Institutes).