Services

We can assist you with the creativity and the experience of our skilled chemists to expand your own R&D effort

A continuous range of problem-solving expertise in chemical synthesis makes C4T an ideal partner for both Process Research & Development and Drug Discovery activities. C4T has set long term partnerships with leading Italian Pharmas for Drug Discovery Services and Process Research & Development. Custom synthesis services are provided to big Pharmas as well.

Organic Synthesis
&
Route Development Go to Section

NMR Studies
&
Chemical Analysis Go to Section

Medicinal Chemistry Go to Section

Organic Synthesis & Route Development

Organic Synthesis

We provide efficient contract synthesis to produce high-quality, high-purity complex organic molecules in milligram to multi-gram quantities:

  • PHARMA & AGRO API IMPURITIES
    • Route development and synthesis of high value and non commercially available API impurities (ansamycines, betalactames, purine nucleosides, ketoprofen, SCMC, Levodropropizine and more)
    • Isolation and structure elucidation of unknown impurities
    • Single point chemical modification ans isotope synthesis for the validation of new strucutres
    • Advanced NMR studies (link to section)
  • Original scaffolds/building blocks
  • Reference compounds

Organic synthesis at C4T accounts for the attitude of problem solving. We long for the most demanding tasks. We explore novel chemistry and synthetic routes by exploiting up-to-date search engines and a distinctive know-how in organic chemistry. We can help you with patents, strategies, evaluation of prior art.
Purification strategies are evaluated on a case-by-case basis including several techniques (e.g., silica/alumina column chromatography, preparative HPLC, ion-exchange, etc.).
Detailed structure elucidation is provided by combining HPLC-MS analysis with complete NMR studies. Most suitable NMR techniques are selected on a case-by-case basis according to complexity of the task. (“NMR Studies & Chemical Analysis” )
Our staff has experience in developing projects up to 100 g scale in our research laboratories, transferring know-how and expertise to our customers and helping them in the first steps of scale-up process.

Documentation

All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.

NMR STUDIES & CHEMICAL ANALYSIS

NMR Studies & Chemical Analysis

These services include:

  • isolation and STRUCTURE ELUCIDATION OF UNKNOWN API IMPURITIES from process mixtures
  • structure elucidation of compounds and mixtures of compounds
  • analytical methods development and validation (ICH guidelines)
  • qNMR for absolute concentration determination of test items (APIs, API impurities, working standards, etc..)
  • analytical characterization of natural and semi-synthetic polysaccharides

High-quality investigations of purity and/or structure of compounds are performed combining several techniques (e.g., NMR, GC-MS, HPLC-MS, HPLC-RID, UV, GPC-RID).

We have developed an Advanced NMR Platform (Bruker 700 / 400 MHz) able to address several tasks, in collaboration with NMR Spectroscopy and Structural Biochemistry Laboratory – “Tor Vegata University” and Prof. M. Paci, who devoted a whole career to NMR studies of biological systems.
The platform includes:

  • Mono-dimensional NMR: 1H; 13C, Heteronuclei
  • Two-dimensional NMR: TOCSY, NOESY, ROESY, HSQC, HMBC
  • qNMR studies
  • Diffusion Ordered Spectroscopy (DOSY) focused on:
    • Mixture Analysis
    • Characterization of Complexes

Recent case studies

Documentation

All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.
Analytical Method Validation Protocols, Validation Protocol Reports, Analytical Method Procedures and Analysis Reports are released according to the specific service provided.

Medicinal Chemistry Tag

Medicinal Chemistry

Organic synthesis
  • Synthesis of focused libraries solution and solid phase parallel synthesis
  • Synthesis of original scaffolds
  • Strategy design for hit expansion and optimisation
  • Scale up and synthesis of lead compounds
Computational studies

We will help you, hand in hand, in developing your hit identification and hit-to-lead programs in close collaboration with Prof. Orazio Nicolotti (University of Bari – Aldo Moro) (link sito Nicolotti)

Documentation

All experiments are recorded with details in our Electronic Lab Notebook. Data are provided in a written report.

C4T has matured great experience in specific research areas (i.e., Endocannabinoids, Metalloproteinases, Kinases) driving and developing early stage discovery projects through the closest cooperation with Biology and Medicine Departments of excellence (Academia/Research Institutes).

Have a look a tour projects to expand your portfolio